【文獻標題】Hyperlipidemia induces typical atherosclerosis development in Ldlr and Apoe deficient rats
【作者】Yongliang Zhao, Yiqing Yang, Roumei Xing，et.al
【作者單位】華東師范大學(xué)（East China Normal University）
【文獻中引用產(chǎn)品】
大鼠胰島素(INS)ELISA試劑盒
大鼠瘦素(LEP)ELISA試劑盒
大鼠游離脂肪酸(FFA)ELISA試劑盒
【關(guān)鍵詞】 Apoe; Ldl receptor; atherosclerosis; rat; gene knockout
【DOI】10.1016/j.atherosclerosis.2018.02.015
【影響因子(IF)】4.26
【出版期刊】《Atherosclerosis》
【產(chǎn)品原文引用】
Biochemical analysis Rats were fasted overnight (12-14 h) and blood samples from the retro-orbital plexus were collected. Serum was obtained by centrifugation at 3000 rpm for 15 min at 4 °C, then kept frozen at ?80 °C until analysis. Lipids and lipoproteins including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), ApoB and lipoprotein (a) were analyzed using AU680 Automatic Biochemistry Analyzer (Beckman Coulter, USA). FPLC lipoprotein profiles were assessed by size-exclusion chromatography on Superose 6 10/300 GL column and AKTA purifier (GE Healthcare) [16]. We also measured liver and kidney indexes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA) and creatinine levels. Furthermore, the atherosclerosis index and LDL/HDL ratio were calculated. Atherosclerosis index was calculated as [TC- HDL-C]/HDL-C [17]. Serum insulin, leptin and free fatty acid (FFA) levels were measured using ELISA kits (Hengyuan Biological Technology Co. Ltd., Shanghai, China
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